Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases alpha and beta

Madoka Watanabe; Shinya Hanashima; Yoshiyuki Mizushina; Hiromi Yoshida; Masahiko Oshige; Kengo Sakaguchi; Fumio Sugawara

doi:10.1016/s0960-894x(01)00725-9

Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases alpha and beta

Bioorg Med Chem Lett. 2002 Feb 11;12(3):287-90. doi: 10.1016/s0960-894x(01)00725-9.

Authors

Madoka Watanabe¹, Shinya Hanashima, Yoshiyuki Mizushina, Hiromi Yoshida, Masahiko Oshige, Kengo Sakaguchi, Fumio Sugawara

Affiliation

¹ Department of Applied Biological Science, Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278-8510, Japan.

PMID: 11814779
DOI: 10.1016/s0960-894x(01)00725-9

Abstract

Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases alpha and beta with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biotin / chemistry
Chromatography, Affinity
DNA Polymerase I / antagonists & inhibitors
DNA Polymerase I / chemistry*
DNA Polymerase I / isolation & purification
DNA Polymerase beta / antagonists & inhibitors
DNA Polymerase beta / chemistry*
DNA Polymerase beta / isolation & purification
Enzyme Inhibitors / chemical synthesis
Enzyme Inhibitors / chemistry*
Enzyme Inhibitors / pharmacology
Indicators and Reagents
Kinetics
Lithocholic Acid / chemical synthesis
Lithocholic Acid / chemistry*
Lithocholic Acid / pharmacology
Magnetic Resonance Spectroscopy
Rats
Spectrometry, Mass, Fast Atom Bombardment

Substances

Enzyme Inhibitors
Indicators and Reagents
Lithocholic Acid
Biotin
DNA Polymerase I
DNA Polymerase beta